Rat Il-23 Potency Assay

Description: (CT) IL-23 peptide

Description: (NT) IL-23 peptide

Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Description: Quantitativesandwich ELISA kit for measuring Human Interleukin 23, IL-23 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Description: Interleukin 6 (IL-6), and its receptor complex interleukin 6 receptor (IL-6R, CD126)-gp130 (CD130), play an important role in immune response. Elevated IL-6 leads to an acute severe systemic inflammatory response known as 'cytokine storm', where an uncontrolled and excessive release of pro-inflammatory cytokines can cause multisystem organ failure and death. Mortality in COVID-19 patients has been linked to this severe response induced by the virus. Tocilizumab is a humanized monoclonal antibody against the IL-6R used as immunosuppressive drug, and it is emerging as an alternative treatment for COVID-19 patients at risk for 'cytokine storm'._x000D_
The IL-6:IL-6R Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this receptor-ligand interaction. The key to this kit is the high sensitivity of detection of IL-6-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, IL-6R protein is attached to a nickel-coated 96-well plate. Next, IL-6-Biotin incubated with IL-6R on the plate. Finally, the plate is treated with Streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.

Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Description: This particular kit is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential(MMP).

Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Description: The IL-17RA[Biotin]:IL-17A Inhibitor Screening Assay Kit is designed for_x000D_screening inhibitors of IL-17A:IL-17RA interaction. The IL-17RA[Biotin]-IL-17A Binding Assay Kit_x000D_comes in a convenient 96-well format, with biotin-labeled IL-17RA, purified IL-17A, streptavidinlabeled_x000D_HRP, and assay buffer for 100 binding reactions. The key to this kit is the high_x000D_sensitivity of detection of biotin-labeled IL-17RA by streptavidin-HRP. Only a few simple steps_x000D_on a microtiter plate are required for the assay. First, IL-17A is coated on a 96-well plate. Next,_x000D_IL-17RA is incubated with IL-17A on the plate. Finally, the plate is treated with streptavidin-HRP_x000D_followed by addition of an HRP substrate to produce chemiluminescence, which can then be_x000D_measured using a chemiluminescence reader.

Description: Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

Description: The IL-2: IL-2 Receptor Alpha (IL-2RA) Inhibitor Screening Colorimetric Assay Kit is designed for the screening and profiling of inhibitors or neutralizing antibodies blocking the interaction between human IL-2 and its receptor IL-2RA. _x000D_The assay requires only a few steps. First, IL-2RA is coated on a 96-well plate overnight. After blocking, the receptor is pre-incubated with the test inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-IL-2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.

Description: Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. Altered activity of PDE4B has been associated with schizophrenia and bipolar disorder. It has also recently been found to modulate cognition, as reduction in PDE4B activity improves memory and long-term plasticity in mouse models._x000D_The Rat PDE4B Assay Kit is designed for identification of inhibitors of rat PDE4B (rPDE4B) using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by rPDE4B to the binding agent._x000D_

Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light._x000D_The Rat PDE4B Assay Kit comes in a convenient 96-well format, with purified rPDE4B enzyme, fluorescently labeled rPDE4B substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Rat PDE4B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for rPDE4B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing rPDE4B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: Phosphodiesterase's (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Rat PDE4D, also known as cAMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility.The Rat PDE4D Assay Kit is designed for identification of inhibitors of Rat PDE4D using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Rat PDE4D to the binding agent.Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearlypolarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.The Rat PDE4D Assay Kit comes in a convenient 96-well format, with purified Rat PDE4D enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Rat PDE4D Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Rat PDE4D reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Rat PDE4D for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Rat PDE7A, also known as high affinity cAMP-specific phosphodiesterase 7A, has been implicated in cardiovascular function and fertility. The Rat PDE7A Assay Kit is designed for identification of inhibitors of Rat PDE7A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Rat PDE7A to the binding agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.The Rat PDE7A Assay Kit comes in a convenient 96-well format, with purified Rat PDE7A enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Rat PDE7A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Rat PDE7A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Rat PDE7A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Rat PDE1B, also known as calmodulin-dependent phosphodiesterase, is a dual specificity phosphodiesterase has been implicated in allergy, asthma, hypertension, and schizophrenia. PDE1B plays an important role in T cell activation and survival, and that PDE1B-targeted therapy might prove beneficial in leukemias and inflammatory disorders._x000D_The Rat PDE1B Assay Kit is designed for identification of inhibitors of Rat PDE1B using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Rat PDE1B to the binding agent._x000D_Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearlypolarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light._x000D_The key to the Rat PDE1B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Rat PDE1B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Rat PDE1B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Description: Description of target: The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISpot Immunoassay;Sensitivity:

Description: Description of target: The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISpot Immunoassay;Sensitivity: